Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC202

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC202
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4g12 Donor: Morell, Canberra Trimmed to Rsa1 at both ends. (CA)18 207 to 242, (CA)9 251 to 268. Bases 339-462 (ie. 3' side of microsatellite) match part of 1e2.
Type	PCR SSR
PCR primers	Forward TTgTgcAcATcAATgTAAgcgg at 8 length 22 bases, Tm 66 Reverse TgcTTTTgTTTgTcgTcgAggT at 414 length 22 bases, Tm 66
SSR size	428 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC202
Source Species	Triticum aestivum