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GrainGenes Probe Report: WMC195

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC195  [ Marker Report ]
Locus	Xwmc195-7B wmc195 wmc195-7B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4c10 Donor: Morell, Canberra Trimmed to insert at both ends (removing mostly Ns). Small microsatellite. (CCG)5 82-96, (CCG)4 108-119.
Type	PCR SSR
PCR primers	Forward AgAgTTcAgATcTAcAAggcgg at 16 length 22 bases, Tm 62 Reverse gggccccAAATTAATTAgcAcc at 212 length 22 bases, Tm 66
SSR size	218 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, product;
Sequence	WMC195
Source Species	Triticum aestivum