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GrainGenes Probe Report: WMC192

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC192
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4b9 Donor: Morell, Canberra Trimmed to Rsa1 at both ends. (CA)10 73 to 92. Bases 1-110 (ie. 5' side of microsatellite) match part of 9D1. Bases 3-97 (ie 5' side of microsatellite) match part of 3a12(1).
Type	PCR SSR
PCR primers	Forward TgTAggAgTAggTcgATATgTc at 22 length 22 bases, Tm 55 Reverse ccTccgTcTAggTgTAATAAgT at 138 length 22 bases, Tm 56
SSR size	138 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product;
Sequence	WMC192
Source Species	Triticum aestivum