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GrainGenes Probe Report: WMC191

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC191
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4g8 Donor: Morell, Canberra Trimmed to insert at 5' end and to Rsa1 at 3' end. (GT)14 102 to 129,
Type	PCR SSR
PCR primers	Forward TgcgcgcATgcTcgAAgAAg at 75 length 20 bases, Tm 72 Reverse ggggAgcggTAcccgcgTcA at 158 length 20 bases, Tm 75
SSR size	103 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC191
Source Species	Triticum aestivum