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GrainGenes Probe Report: WMC189

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC189
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4c8 Donor: Morell, Canberra Trimmed to Rsa1 at 5' end, and to insert at 3' end (removing mostly Ns).(GT)8 74 to 89,
Type	PCR SSR
PCR primers	Forward gTcAAATcgggggcgTcAcAAA at 6 length 22 bases, Tm 72 Reverse gTgAcAcgTcgATcTggATAAT at 215 length 22 bases, Tm 61
SSR size	231 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC189
Source Species	Triticum aestivum