Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC188

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC188  [ Marker Report ]
Locus	Xwmc188
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4b8 Donor: Morell, Canberra Trimmed to insert at both ends (removing Ns). Multiple inserts (2) in this clone, joined at 277-294 by linkers. (CA)9 137 to 154. Second insert is a member of 1g1 family.
Type	PCR SSR
PCR primers	Forward cgcgTggAATTAgATgAcccTT at 3 length 22 bases, Tm 66 Reverse ccTTTgcTTccgATcAAATgTg at 237 length 22 bases, Tm 65
SSR size	256 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC188
Source Species	Triticum aestivum