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GrainGenes Probe Report: WMC186

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC186
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4f7 Donor: Morell, Canberra Trimmed to insert at both ends (mostly Ns removed). (CA)8 152 to 167.
Type	PCR SSR
PCR primers	Forward cAAgTccAgTccTcTTAggcAc at 31 length 22 bases, Tm 62 Reverse TccgTATgggcgTAATAAgTcA at 203 length 22 bases, Tm 63
SSR size	194 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC186
Source Species	Triticum aestivum