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GrainGenes Probe Report: WMC185

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC185
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4e7 Donor: Morell, Canberra Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 125 bases located on 4A, 129 bases located on 4B, 272 bases located on 3B, 314 bases located on 5A, 418 bases located on 4A, 555 bases located on 4A Trimmed to insert at 5' end and to Rsa1 at 3' end. Small microsatellite (GGC)3A(GGC)3 at 123-141.
Type	PCR SSR
PCR primers	Forward ggggATTggAgTggTggAgAgA at 47 length 22 bases, Tm 68 Reverse cATTcggAAATcAggAccgTgg at 153 length 22 bases, Tm 69
SSR size	129 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC185
Source Species	Triticum aestivum