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GrainGenes Probe Report: WMC183

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC183  [ Marker Report ]
Locus	Xwmc183 Xwmc183-1A wmc183 wmc183-1A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 9h6 Donor: Bernard, Clermont-Ferrand Trimmed to insert at 5' and and to Rsa1 at 3' end. Bases 266-347 (complement) ie. 3' side of microsatellite match part of 11g3. Bases 1-290 (ie 5' side of microsatellite) match part of 3c12. (CA)18 243 to 278.
Type	PCR SSR
PCR primers	Forward cAgAAAcggcTcAAcTTAAcAA at 95 length 22 bases, Tm 61 Reverse TcTgATcTcgTgATcAgAATAg at 297 length 22 bases, Tm 56
SSR size	224 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide.; 51C anneal, smear;
Sequence	WMC183
Source Species	Triticum aestivum