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GrainGenes Probe Report: WMC182

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC182  [ Marker Report ]
Locus	Xwmc182-7A [ Show all 19 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 9g6 Donor: Bernard, Clermont-Ferrand Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 165 bases located on 7B Trimmed to Rsa1 at both ends. (CT)16 194 to 225.
Type	PCR SSR
PCR primers	Forward gTATcTcAcgAgcATAAcAcAA at 91 length 22 bases, Tm 57 Reverse gAAAgTgTATggATcATTAggc at 228 length 22 bases, Tm 57
SSR size	159 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC182
Source Species	Triticum aestivum