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GrainGenes Probe Report: WMC181

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC181  [ Marker Report ]
Locus	Xwmc181-2A [ Show all 10 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 9d6 Donor: Bernard, Clermont-Ferrand Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 260 bases located on 2A Trimmed to insert at both ends. (GT)19 126 to 163, (GT)10 166 to 185.
Type	PCR SSR
PCR primers	Forward TccTTgAccccTTgcAcTAAcT at 20 length 22 bases, Tm 64 Reverse ATggTTgggAgcAcTAgcTTgg at 258 length 22 bases, Tm 66
SSR size	260 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC181
Source Species	Triticum aestivum