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GrainGenes Probe Report: WMC178

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC178
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 9e5 Donor: Bernard, Clermont-Ferrand Trimmed to Rsa1 at 5' end and to insert at 3' end. (GT)14 125 to 152. Bases 178-281 (ie. 3' side of microsatellite) match part of 1B3 . Bases 183 to 216 show homology to 25h3 bases 202 to 235 (34 in longest run, maxScore = 83).
Type	PCR SSR
PCR primers	Forward ccAcTTAgAAAccAcAccTgcT at 92 length 22 bases, Tm 62 Reverse AcgTccggcAcTAcTTcATAcT at 269 length 22 bases, Tm 62
SSR size	199 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC178
Source Species	Triticum aestivum