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GrainGenes Probe Report: WMC175

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC175  [ Marker Report ]
Locus	Xwmc175-2B [ Show all 13 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 9b4 Donor: Bernard, Clermont-Ferrand Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 135 bases located on 2B, 145 bases located on 2B, 181 bases located on 2B, 256 bases located on 2B Trimmed to insert at 5' end and to Rsa1 at 3' end. (CA)16 138 to 169.
Type	PCR SSR
PCR primers	Forward gcTcAgTcAAAccgcTAcTTcT at 17 length 22 bases, Tm 62 Reverse cAcTAcTccAATcTATcgccgT at 248 length 22 bases, Tm 62
SSR size	253 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC175
Source Species	Triticum aestivum