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GrainGenes Probe Report: WMC171

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC171  [ Marker Report ]
Locus	Xwmc171
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 9c3 Donor: Bernard, Clermont-Ferrand Trimmed to Rsa1 at both ends. (GA)15 178 to 207, (GT)10 158 to 177.
Type	PCR SSR
PCR primers	Forward AgTgTgcggTgTcTAAccTATc at 22 length 22 bases, Tm 60 Reverse ATgTTTgTTgcggTcTccTTTc at 275 length 22 bases, Tm 64
SSR size	275 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC171
Source Species	Triticum aestivum