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GrainGenes Probe Report: WMC169

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC169
Locus	Xwmc169-3A [ Show all 7 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 9g2 Donor: Bernard, Clermont-Ferrand Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 127 bases located on 3D, 171 bases located on 3A Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)25 65 to 114.
Type	PCR SSR
PCR primers	Forward TAcccgAATcTggAAAATcAAT at 2 length 22 bases, Tm 61 Reverse TggAAgcTTgcTAAcTTTggAg at 147 length 22 bases, Tm 62
SSR size	167 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC169
Source Species	Triticum aestivum