Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC168

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC168  [ Marker Report ]
Locus	Xwmc168-7A Xwmc168 wmc168
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 9d2 Donor: Bernard, Clermont-Ferrand Trimmed to Rsa1 site at 5' end, and to insert at 3' end. (CT)27 338 to 391. Big difference between Tms of primers.
Type	PCR SSR
PCR primers	Forward AAcAcAAAAgATccAAcgAcAc at 91 length 22 bases, Tm 63 Reverse cAgTATAgAAggATTTTgAgAg at 388 length 22 bases, Tm 52
SSR size	319 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC168
Source Species	Triticum aestivum