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GrainGenes Probe Report: WMC167

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC167  [ Marker Report ]
Locus	Xwmc167-2D [ Show all 6 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 9b2 Donor: Bernard, Clermont-Ferrand Trimmed to insert at both ends. Multiples inserts (2) in this clone separated 380 -397 by linkers. (CA)22 87 to 130, (CA)8 535 to 550, (CA)8 553 to 568. Primers are for first insert in this clone.
Type	PCR SSR
PCR primers	Forward AgTggTAATgAggTgAAAgAAg at 7 length 22 bases, Tm 57 Reverse TcggTcgTATATgcATgTAAAg at 170 length 22 bases, Tm 59
SSR size	185 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC167
Source Species	Triticum aestivum