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GrainGenes Probe Report: WMC166

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC166  [ Marker Report ]
Locus	Xwmc166-7B [ Show all 8 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 9g1 Donor: Bernard, Clermont-Ferrand Trimmed to Rsa1 at 5' end and to insert at 3' end. (GA)8 347 to 362, (GT)8 297 to 312, (GT)8 327 to 342. (Near) Duplicate of first insert in 3E8. Bases 60 to 310 show homology to 14f6 bases 1 to 251 (251 in longest run, maxScore = 183).
Type	PCR SSR
PCR primers	Forward ATAAAgcTgTcTcTTTAgTTcg at 81 length 22 bases, Tm 54 Reverse gTTTTAAcAcATATgcATAccT at 364 length 22 bases, Tm 53
SSR size	305 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC166
Source Species	Triticum aestivum