Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC165

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC165
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 9e1 Donor: Bernard, Clermont-Ferrand Trimmed to insert at 5' end and to insert at 3' end. (GT)9 53 to 70. Bases 40-141 ie to 3' side of microsatellite matches 4b10. Bases 3-67 (complement) ie. 5' side of microsatellite match part of 1d11.
Type	PCR SSR
PCR primers	Forward cAcAcTcgcAcgATTTTccTAT at 4 length 22 bases, Tm 63 Reverse TcggTTAcAcTggAAgTggTcT at 192 length 22 bases, Tm 63
SSR size	210 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide; 51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC165
Source Species	Triticum aestivum