Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC162

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC162
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11g6 Donor: Rogers, Monsanto AGR204.47.SEQ. Trimmed to insert at 5' end and to Rsa1 at 3' end. (TG)5TA(TG)5 at 210-230. Matches 11f7
Type	PCR SSR
PCR primers	Forward AcTcAAggcAgccTgggTATgg at 56 length 22 bases, Tm 68 Reverse cccccgTcATTTccTcAAAcAc at 248 length 22 bases, Tm 68
SSR size	214 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 51C anneal, smear;
Sequence	WMC162
Source Species	Triticum aestivum