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GrainGenes Probe Report: WMC159

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC159
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11d6 Donor: Rogers, Monsanto AGR204.44.SEQ. Trimmed to insert at 5' and to Rsa1 at 3' end. (GT)6T(GT)6 at 145-161. Bases 62 to 82 show homology to 2h1.1 bases 145 to 165 (complement) (21 in longest run, maxScore = 36).
Type	PCR SSR
PCR primers	Forward cggggAccTTgcTAgggTTTcA at 63 length 22 bases, Tm 70 Reverse TTTccTcAAAcAccccATgcTc at 170 length 22 bases, Tm 67
SSR size	129 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC159
Source Species	Triticum aestivum