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GrainGenes Probe Report: WMC156

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC156  [ Marker Report ]
Locus	Xwmc156-1B Xwmc156.2 wmc156-1B wmc156
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11h4 Donor: Rogers, Monsanto Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 237 bases located on 1B AGR204.32.SEQ. Trimmed to insert at both ends. Multiple inserts in this clone joined 230-237 by linkers. (CA)10 30 to 49, (GCC)6 317 to 334. Primers shown here are for first insert. Second insert has complex repeating structure - use as minisatellite probe? Complex band pattern.
Type	PCR SSR
PCR primers	Forward gccTcTAgggAgAAAAcTAAcA at 6 length 22 bases, Tm 58 Reverse TcAAgATcATATccTccccAAc at 195 length 22 bases, Tm 62
SSR size	211 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product;
Sequence	WMC156
Source Species	Triticum aestivum