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GrainGenes Probe Report: WMC155

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC155  [ Marker Report ]
Locus	Xwmc155b
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11g4 Donor: Rogers, Monsanto Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 125 bases located on 3B, 160 bases located on 4B, 190 bases located on 3B AGR204.31.SEQ. Trimmed to insert at both ends. (CCG)5 microsatellite. Weak homology to 13d3.
Type	PCR SSR
PCR primers	Forward TAgTcTTTccTcATTcTccccc at 76 length 22 bases, Tm 62 Reverse cgcggAAgAAgAAgcAcTTAcA at 249 length 22 bases, Tm 65
SSR size	195 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC155
Source Species	Triticum aestivum