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GrainGenes Probe Report: WMC151

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC151
Locus	Xwmc151
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11c3 Donor: Rogers, Monsanto AGR204.19.SEQ. Trimmed to insert at 5' end and to Rsa1 at 3' end.(CA)30 188 to 247. 2 loci, 1 weak polymorphic of correct size. Bases 1-216 (complment) ie 5' side of microsatellite match part of 7e4.
Type	PCR SSR
PCR primers	Forward TAcggccTAgAAgATATgAgcA at 19 length 22 bases, Tm 61 Reverse TTgTTgTTTTggAAcgcTTgTc at 264 length 22 bases, Tm 64
SSR size	267 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC151
Source Species	Triticum aestivum