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GrainGenes Probe Report: WMC148

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC148
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11g1 Donor: Rogers, Monsanto AGR204.7.SEQ. Trimmed to Rsa 1 at 5' end, and to insert at 3' end. Identical to 11g2.(CT)15 86 to 115. (3 loci - 1 ploymorphic but weak). 3' side of microsatellite matches 5g11. Bases 73 to 115 show homology to 25e10 bases 65 to 107 (complement) (43 in longest run, maxScore = 35).
Type	PCR SSR
PCR primers	Forward ccgATgAAgcgTgAgTAgTTTA at 23 length 22 bases, Tm 61 Reverse gATcTcAAcAAAcAcAcTgccA at 174 length 22 bases, Tm 63
SSR size	173 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC148
Source Species	Triticum aestivum