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GrainGenes Probe Report: WMC146

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC146
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 5b12 Donor: Henry, Lismore (CA)31 137 to 198. Bases 243-322 ie. 3' side of microsatellite match 5g10, bases 148-331(ie 3' side of microsatellite) match part of 5F2.
Type	PCR SSR
PCR primers	Forward ccAAgTggTTcTTTccATcATc at 115 length 22 bases, Tm 62 Reverse gTgggTAAAgTgTgcAAccTcT at 292 length 22 bases, Tm 62
SSR size	199 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC146
Source Species	Triticum aestivum