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GrainGenes Probe Report: WMC145

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC145  [ Marker Report ]
Locus	Xwmc145-6A wmc145
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 5d11 Donor: Henry, Lismore Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 125 bases located on 1D, 140 bases located on 6A, 210 bases located on 1D, 290 bases located on 6A, 301 bases located on 1B, 310 bases located on 2A complex small microsatellite GTGGCCG (CGG)3TGG(CGG)2C at 77 - 102. Matches 5C6.
Type	PCR SSR
PCR primers	Forward ggcggTgggTTcAAgTcgTcTg at 40 length 22 bases, Tm 72 Reverse ggAcgAgTcgcTgTccTccTgg at 225 length 22 bases, Tm 72
SSR size	207 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC145
Source Species	Triticum aestivum