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GrainGenes Probe Report: WMC143

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC143  [ Marker Report ]
Locus	Xwmc143
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 5g9 Donor: Henry, Lismore (CA)9 206 to 223, (CA)19 234 to 271. (2 loci). Bases 108-223 (ie. 5' side of microsatellite) match 11c5. Bases 240 to 415 show homology to 2f4 bases 103 to 278 (176 in longest run, maxScore = 124).
Type	PCR SSR
PCR primers	Forward cTTgAcccAAgTAgTTcTTTcc at 177 length 22 bases, Tm 59 Reverse gTgggTAAAgTgTgcAAccTcT at 367 length 22 bases, Tm 62
SSR size	212 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product;
Sequence	WMC143
Source Species	Triticum aestivum