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GrainGenes Probe Report: WMC142

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC142
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 5c9 Donor: Henry, Lismore Complex small microsatellite motif (CG)2(ACTC)2(AC)2(GC)6 at 296-328. Microsatellite is buried in domains of larger repeats (4 repeats 5', 2 repeats 3'), take care if designing new primers.
Type	PCR SSR
PCR primers	Forward AAccgcAAcTcTTgTgAccgTg at 231 length 22 bases, Tm 68 Reverse gcTAgAccTgccTgTcggTATg at 352 length 22 bases, Tm 65
SSR size	143 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, product;
Sequence	WMC142
Source Species	Triticum aestivum