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GrainGenes Probe Report: WMC141

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
WMC141  [ Marker Report ]
Locus
Xwmc141
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 5a9
Donor: Henry, Lismore
Trimmed to insert at 3' end (CA)15 66 to 95. Complex.
Type
PCR
SSR
PCR primers
Forward TgcAAggggTTgAgTTcTTAgg at 40 length 22 bases, Tm 64
Reverse TTgATAgAgcAAgAcgAAgggc at 117 length 22 bases, Tm 65
SSR size
99 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC141
Source Species
Triticum aestivum