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GrainGenes Probe Report: WMC140

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC140
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 5d8 Donor: Henry, Lismore (GT)12 78 to 101.
Type	PCR SSR
PCR primers	Forward cccTcTccTcgggTgTTgcTTg at 52 length 22 bases, Tm 71 Reverse cccAggAgcccTcATgcATAcg at 130 length 22 bases, Tm 72
SSR size	100 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear; 51C anneal, product;
Sequence	WMC140
Source Species	Triticum aestivum