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GrainGenes Probe Report: WMC139

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC139  [ Marker Report ]
Locus	Xwmc139-7A wmc139
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 5a8 Donor: Henry, Lismore Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 66 bases located on 7A (CA)12 144 to 167.
Type	PCR SSR
PCR primers	Forward TgTAAcTgAgggccATgAAT at 121 length 20 bases, Tm 60 Reverse cATcgAcTcAcAAcTAgggT at 57 length 20 bases, Tm 57
SSR size	66 bases expected, Chinese Spring
Amplification Conditions	51C anneal, smear;
Sequence	WMC139
Source Species	Triticum aestivum