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GrainGenes Probe Report: WMC138

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC138
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 5f7 Donor: Henry, Lismore (CA)14 172 to 199, (GA)8 200 to 215. Bases 80-180 (complement) ie 5' side of microsatellite match 3' side microsatellite in 7H8.
Type	PCR SSR
PCR primers	Forward AATcccggTcAAATgAgAAT at 2 length 20 bases, Tm 60 Reverse gAgAcATgcATgAATcAgAA at 300 length 20 bases, Tm 57
SSR size	318 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product;
Sequence	WMC138
Source Species	Triticum aestivum