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GrainGenes Probe Report: WMC136

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC136
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 5A7 Donor: Henry, Lismore Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 187 bases located on 4A, 190 bases located on 5D, 198 bases located on 5B Microsatellite motif consists of (AGGCGG)4 at 68-96. Several products one of correct size. Bases 3 to 110 show homology to 15d3 bases 252 to 359 (108 in longest run, maxScore = 186).
Type	PCR SSR
PCR primers	Forward gTATgATgAcAcATggTgcTgc at 12 length 22 bases, Tm 63 Reverse gAccTgATTcTgcTgTgcTcTg at 188 length 22 bases, Tm 64
SSR size	198 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC136
Source Species	Triticum aestivum