Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC135

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC135
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7h12 Donor: Blake, Montana Microsatellite: (AC)~30. Tom Primer 1 : GAAGC TATAC AGTTA TCATC (35%) Tom Primer 2 : GGATG GGGAT GAATT GTGTG (50%) EXPECTED PCR PRODUCT : ABT. 170 nts. Size of microsatellite is approximate.(CA)29 95 to 152. Bases 190-232 homologous to part of 7a2. May expect secondary fragment of 77 bases.
Type	PCR SSR
PCR primers	Forward cAAccATcAAcAgccAcAcAgc at 14 length 22 bases, Tm 68 Reverse gccATATAgccTTggATggggA at 202 length 22 bases, Tm 68
SSR size	210 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product (slightly stuttery) at 80 bases
Sequence	WMC135
Source Species	Triticum aestivum