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GrainGenes Probe Report: WMC134

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC134  [ Marker Report ]
Locus	Xwmc134 Xwmc134-1B wmc134 wmc134-1B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7c12 Donor: Blake, Montana Microsatellite : [TG]18. Tom Primer 1 : CCAAG CTGTC TGACT GCCAT (55%). Tom Primer 2 : TATAG ACCTC TGGCT CACGG (55%). TOM's EXPECTED PCR PRODUCT: 135 nts(GT)17 161 to 194.
Type	PCR SSR
PCR primers	Forward ccAAgcTgTcTgAcTgccATAg at 86 length 22 bases, Tm 64 Reverse AgTATAgAccTcTggcTcAcgg at 201 length 22 bases, Tm 61
SSR size	137 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide (152-174 bases).
Sequence	WMC134
Source Species	Triticum aestivum