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GrainGenes Probe Report: WMC132

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC132
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7a12 Donor: Blake, Montana Tom Primer 1 : GCCTA GATGA CTCCA CGAAC (55%) (GT)34 276 to 343, (CT)32 401 to 464. Expected fragment size is estimate.
Type	PCR SSR
PCR primers	Forward cTAggTcAcATTgcggcgAAgT at 235 length 22 bases, Tm 67 Reverse gAcggccAggAggATTATcAgT at 379 length 22 bases, Tm 65
SSR size	166 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product.
Sequence	WMC132
Source Species	Triticum aestivum