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GrainGenes Probe Report: WMC131

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC131
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7h11 Donor: Blake, Montana Microsatellite shown here is (AT)10(GA)10, in the original sequence file was marked [AT]N[GA]N. (GA)10 80 to 99, (TA)9 61 to 78, Bases 285 to 317 show homology to 12a2 bases 207 to 239 (33 in longest run, maxScore = 28).
Type	PCR SSR
PCR primers	Forward gcAAcgTcATgcTccgATAAc at 30 length 21 bases, Tm 65 Reverse gcATccAgTccTTcTTcgcTT at 200 length 21 bases, Tm 65
SSR size	191 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product.
Sequence	WMC131
Source Species	Triticum aestivum