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GrainGenes Probe Report: WMC130

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
WMC130
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 7g11
Donor: Blake, Montana
Microsatellite : [GT]32. Tom Primer 1 : GAGGA GAGAT AGAAC AGATC (45%). Tom Primer 2 : TTGTC CATTG GTTCA CAAAC (40%)EXPECTED PCR PRODUCT: 136 nts.(GT)32 384 to 447.
Type
PCR
SSR
PCR primers
Forward TgTggTgccAAcTTcTTcTATg at 281 length 22 bases, Tm 62
Reverse cTccgTgAcTTcTcTcTcAAAA at 498 length 22 bases, Tm 60
SSR size
239 bases expected, Chinese Spring
Amplification Conditions
tested 61C anneal, no product; tested 51C anneal, no product.
Sequence
WMC130
Source Species
Triticum aestivum