Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC129

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC129
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7f11 Donor: Blake, Montana Microsatellite : [CA]12. Tom Primer 1: TAACG TGGGC TGGCT AGGGT (60%). Tom Primer 2: CTACG TCTCG GCGCC CGGAG (75%)EXPECTED PCR PRODUCT: 150 nts.(CA)12 111 to 134, bases 110-217 3' side of microsatellite match 2a10. Bases 138-205 (ie 3' side of microsatellite) match part of 11f10 (complement), reverse primer is in this duplicated region.
Type	PCR SSR
PCR primers	Forward TgAgAgccgAccTccTcTTAcT at 61 length 22 bases, Tm 63 Reverse cggAgAcggTcgAcTggATTAT at 134 length 22 bases, Tm 66
SSR size	95 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product (78, 150 and 290 bases)
Sequence	WMC129
Source Species	Triticum aestivum