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GrainGenes Probe Report: WMC128

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC128  [ Marker Report ]
Locus	Xwmc128-1B wmc128 wmc128-1B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7c10 Donor: Blake, Montana Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 207 bases located on 1B Microsatellite : [GT]16 [GA]10. Tom Primer 1 : CGAAT CGGAC AGCTA CTGCT (55%). Tom Primer 2 : CACCC TTGTG TGTCC ACGTT (55%). EXPECTED PCR PRODUCT: 134 nts. (GA)10 195 to 214, (GT)16 163 to 194. Bases 163-291 (ie. 3' side of microsatellite) homologous to part of 2g10 (reverse primer in this domain).
Type	PCR SSR
PCR primers	Forward cggAcAgcTAcTgcTcTccTTA at 73 length 22 bases, Tm 63 Reverse cTgTTgcTTgcTcTgcAcccTT at 250 length 22 bases, Tm 68
SSR size	199 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product (202 bases).
Sequence	WMC128
Source Species	Triticum aestivum