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GrainGenes Probe Report: WMC127

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC127
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7g9 Donor: Blake, Montana Microsatellite : [CA]9 Tom Primer 1 : AATCT CTATC AACTC CTCTT (35%). Tom Primer 2 : TTGAC AGATT AAGAT TAGAT (25%). EXPECTED PCR PRODUCT: 166 nts. Bases 173-255 (complement) have homology to 8c12(1). (CA)8 50 to 65, (CA)9 140 to 157.
Type	PCR SSR
PCR primers	Forward TTgAcTAAcgggTTTAccTggc at 2 length 22 bases, Tm 64 Reverse gTTgAccTTcTgcTgTcATTgc at 238 length 22 bases, Tm 64
SSR size	258 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, high MW product.
Sequence	WMC127
Source Species	Triticum aestivum