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GrainGenes Probe Report: WMC125

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC125  [ Marker Report ]
Locus	Xwmc125-4B Xwmc125 wmc125
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7g8 Donor: Blake, Montana Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 256 bases located on 4B Microsatellite : [TA]5 TGC [GT]11 GA [GT]20 Tom Primer 1 : AGTTG ACTTC ATATG TTAGG (35%) Tom Primer 2 : TTATC GAAGG ACTAC GATAG (40%) EXPECTED PCR PRODUCT: 181 nts.(GT)11 213 to 234, (GT)20 237 to 276,
Type	PCR SSR
PCR primers	Forward ATAccAccATgcATgTggAAgT at 138 length 22 bases, Tm 63 Reverse AccgcTTgTcATTTccTTcTgT at 367 length 22 bases, Tm 64
SSR size	251 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide (alleles 234-250).
Sequence	WMC125
Source Species	Triticum aestivum