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GrainGenes Probe Report: WMC124

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC124
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7g7 Donor: Blake, Montana 7G7 Microsatellite : [TG]14. Tom PRIMER 1 : GCGGT CGACT GGATT ATGAG (55%). Tom PRIMER 2 : CGAAC TCTAT ATAAG CCACC (45%) EXPECTED PCR PRODUCT: 105 nts. (GT)14 65 to 92. Bases 64-185 (complement) ie 3' side of microsatellite match part of 2a10.
Type	PCR SSR
PCR primers	Forward cggAggcggTcgAcTggATT at 37 length 20 bases, Tm 71 Reverse gTgcccAgcAgggAcgAggA at 186 length 20 bases, Tm 72
SSR size	169 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear.
Sequence	WMC124
Source Species	Triticum aestivum