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GrainGenes Probe Report: WMC123

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC123
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7b7 Donor: Blake, Montana 7B7 Microsatellite : [ AC ]17 Tom Primer1 : GGGTA GGTCG ATGTG TCGCT (60%) Tom Primer 2 : TGGAT GGTAA CATGC ATGTC (45%) EXPECTED PCR PRODUCT : 138 nts.(CA)16 56 to 87. Duplicate 7b10. Bases 1-88 (ie 5' side of microsatellite) match part of 6c6 forward primer incoorporates 3 mismatches between sequences.
Type	PCR SSR
PCR primers	Forward TgTAggggTAggTcgATgTgTc at 5 length 22 bases, Tm 63 Reverse ggATggTAAcATgcATgTcTTc at 123 length 22 bases, Tm 61
SSR size	140 bases expected, Chinese Spring
Amplification Conditions	61C anneal, stuttery product.
Sequence	WMC123
Source Species	Triticum aestivum