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GrainGenes Probe Report: WMC122

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC122
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 8h12 Donor: Flore, Verneuil AGR203. Trimmed to Rsa1 sites at both ends. (CA)22 87 to 130, (CA)12 151 to 174.
Type	PCR SSR
PCR primers	Forward cAgAATTTcTcgTAgAAccA at 22 length 20 bases, Tm 55 Reverse ggcATTgTTgTTAAAgcAcT at 209 length 20 bases, Tm 57
SSR size	207 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product;
Sequence	WMC122
Source Species	Triticum aestivum