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GrainGenes Probe Report: WMC119

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC119
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 8a12 Donor: Flore, Verneuil AGR196. Trimmed to insert at 3' end and to Rsa1 at 5' end. Duplicate 8b12, close relative 1c4. Homologous to reverse of first 71 bases of 11c7 (CA)17 47 to 80. Bases 123 to 162 show homology to 10b10 bases 1 to 40 (complement) (40 in longest run, maxScore = 48). Bases 187 to 225 show homology to 22a6 bases 69 to 107 (complement) (39 in longest run, maxScore = 78).
Type	PCR SSR
PCR primers	Forward TgTgTgTTgTgAgAcgggATTg at 6 length 22 bases, Tm 66 Reverse gccAccTATgTAggTggATgTT at 113 length 22 bases, Tm 62
SSR size	129 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC119
Source Species	Triticum aestivum