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GrainGenes Probe Report: WMC116

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC116  [ Marker Report ]
Locus	Xwmc116-7A Xwmc116 wmc116
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 8e11 Donor: Flore, Verneuil AGR192. Trimmed to insert at 5' end and to Rsa1 at 3' end. Microsatellite core identical to 7a4, 7f12, 11d10, otherwise OK. (CA)13 184 to 209, (CA)8 212 to 227, (CT)12 160 to 183. ( 2-3 loci - at least 2 polymorphic).
Type	PCR SSR
PCR primers	Forward AcggcAccccAgAAccTAgTcg at 71 length 22 bases, Tm 70 Reverse gcgTgcgTcTgTgcTgcATcgg at 435 length 22 bases, Tm 77
SSR size	386 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC116
Source Species	Triticum aestivum