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GrainGenes Probe Report: WMC114

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC114
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 8h10 Donor: Flore, Verneuil AGR187. Trimmed to Rsa1 at 3' end. (CA)12 52 to 75. Polymorphic bands are weak. No bands of predicted size. RAPD like - redesign primers? Original primers contained corrupted plasmid sequence (AAcgAcggccAgTgAATTccTc at 20 , tm68 forward ) - no room to redesign - use at your peril.
Type	PCR SSR
PCR primers	Forward AAcgAcggccAgTgAATTccTc at 20 length 22 bases, Tm 68 Reverse AgcATcgAcATgcAAcAAcccc at 98 length 22 bases, Tm 70
SSR size	100 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC114
Source Species	Triticum aestivum