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GrainGenes Probe Report: WMC110

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC110  [ Marker Report ]
Locus	Xwmc110-5A wmc110 wmc110-5A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 8c8 Donor: Flore, Verneuil Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 172 bases located on 5A AGR166. Reverse sequence only from Lark. Trimmed to insert 5' and Rsa1 at 3'. (GT)11 232 to 253.
Type	PCR SSR
PCR primers	Forward gcAgATgAgTTgAgTTggATTg at 108 length 22 bases, Tm 61.0 Reverse gTAcTTggAAAcTgTgTTTggg at 256 length 22 bases, Tm 60.0
SSR size	170 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC110
Source Species	Triticum aestivum